5XK0
Structure of 8-mer DNA2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-10 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 42.012, 42.012, 25.016 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.707 - 1.451 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.19560 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5wsp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.919 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.500 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.110 | 0.358 |
| Number of reflections | 4199 | 358 |
| <I/σ(I)> | 82.3 | 12.9 |
| Completeness [%] | 98.6 | 87.5 |
| Redundancy | 19.8 | 13.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 10% v/v MPD, 40mM Sodium cacodylate pH 6.0, 12mM Spermine tetra-HCl, 80mM KCl, 20mM MgCl2 |
