Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.56418 |
| Spacegroup name | P 32 1 2 |
| Unit cell lengths | 48.309, 48.309, 83.636 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.576 - 2.097 |
| R-factor | 0.2275 |
| Rwork | 0.225 |
| R-free | 0.24570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xew |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.764 |
| Data scaling software | SCALEPACK |
| Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 2.180 |
| High resolution limit [Å] | 2.097 | 4.520 | 2.100 |
| Rmerge | 0.055 | 0.048 | 0.267 |
| Number of reflections | 12596 | ||
| <I/σ(I)> | 24.8 | ||
| Completeness [%] | 99.6 | 98.1 | 97.8 |
| Redundancy | 6.1 | 6 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 1.0 mM ssDNA, 2.0 mM Chromomycin A3, 4mM CoCl2.6H2O, 50 mM Sodium Cacodylate, 5 mM Spermine, 3% MPD |
