5XJU

Crystal Structure of the Gemin2-binding domain of SMN, Gemin2dN39 in Complex with SmD1(1-82)/D2.R61A/F/E/G from Human

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-12-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	82.780, 112.960, 130.750
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.770 - 2.580
R-factor	0.2082
Rwork	0.205
R-free	0.25820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3S6N
RMSD bond length	0.015
RMSD bond angle	1.864
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	66.770
High resolution limit [Å]	2.580
Number of reflections	31276
<I/σ(I)>	13.5
Completeness [%]	99.9
Redundancy	12.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1% PEG8000, 100mM Tris.HCl