5XJT

Crystal Structure of the Gemin2-binding domain of SMN, Gemin2 in Complex with SmD1(1-82)/D2.R61A/F/E/G from Human

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97846
Spacegroup name	P 21 21 21
Unit cell lengths	82.640, 84.760, 114.330
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	66.980 - 2.920
R-factor	0.2027
Rwork	0.199
R-free	0.28060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3S6N
RMSD bond length	0.010
RMSD bond angle	1.420
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall
Low resolution limit [Å]	66.980
High resolution limit [Å]	2.920
Number of reflections	13784
<I/σ(I)>	8.6
Completeness [%]	99.6
Redundancy	12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1% PEG8000, 100mM Tris.HCl