5XJG

Crystal structure of Vac8p bound to Nvj1p

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	84.082, 115.630, 84.131
Unit cell angles	90.00, 114.68, 90.00

Refinement procedure

Resolution	29.595 - 2.400
R-factor	0.1907
Rwork	0.190
R-free	0.22240
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.908
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.400
Rmerge	0.128
Number of reflections	57806
<I/σ(I)>	13.3
Completeness [%]	98.8
Redundancy	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG400, BTP, t-butanol