5XIW

Crystal structure of T2R-TTL-Colchicine complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-05
Detector	PSI PILATUS 6M
Wavelength(s)	0.97853
Spacegroup name	P 21 21 21
Unit cell lengths	105.491, 159.148, 181.702
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	119.720 - 2.900
R-factor	0.20604
Rwork	0.204
R-free	0.25279
RMSD bond length	0.007
RMSD bond angle	1.245
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall
Low resolution limit [Å]	119.720
High resolution limit [Å]	2.900
Number of reflections	65289
<I/σ(I)>	15.5
Completeness [%]	100.0
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.7	293	6% polyethylene glycol 4000, 8% glycerol, 0.1 M MES, 30 mM CaCl2, 30 mM MgCl2, pH 6.7