5XHY

BRD4 bound with compound Bdi1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	33.839, 47.599, 77.859
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.035 - 1.976
R-factor	0.2151
Rwork	0.211
R-free	0.28510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.282
Data scaling software	HKL-2000
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.060	1.854
High resolution limit [Å]	1.790	1.790
Number of reflections	8186
<I/σ(I)>	12.21
Completeness [%]	99.9
Redundancy	14

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	20% PEG3350, 0.2M NaNO3, 0.1M HEPES, 10% EtGhly, PH 6.5