5XHO

Crystal structure of Frog M-ferritin E135K mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2016-01-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	184.120, 184.120, 184.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.260 - 1.730
R-factor	0.16478
Rwork	0.164
R-free	0.18297
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ka3
RMSD bond length	0.013
RMSD bond angle	1.080
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	21.260	1.760
High resolution limit [Å]	1.730	1.730
Number of reflections	29921
<I/σ(I)>	19.2	4.8
Completeness [%]	99.9	100
Redundancy	12	10.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	2.0 M MgCl2 and 100 mM Bicine (pH 9.0)