5XHM

Crystal structure of Frog M-ferritin D40A mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	103
Detector technology	IMAGE PLATE
Collection date	2016-01-11
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	F 4 3 2
Unit cell lengths	184.170, 184.170, 184.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.980 - 1.700
R-factor	0.15779
Rwork	0.156
R-free	0.18735
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ka3
RMSD bond length	0.009
RMSD bond angle	1.105
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.980	1.730
High resolution limit [Å]	1.700	1.700
Number of reflections	29984
<I/σ(I)>	30.7	6.3
Completeness [%]	100.0	100
Redundancy	13	11.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	277	2.0 M MgCl2 and 100 mM Bicine (pH 9.0)