5XHI
Crystal structure of Frog M-ferritin D38A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 103 |
| Detector technology | CCD |
| Collection date | 2016-04-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.8266 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 184.090, 184.090, 184.090 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.120 - 1.260 |
| R-factor | 0.12676 |
| Rwork | 0.125 |
| R-free | 0.15287 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ka3 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.317 |
| Data reduction software | iMOSFLM (7.1.1) |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.120 | 1.280 |
| High resolution limit [Å] | 1.260 | 1.260 |
| Number of reflections | 72072 | |
| <I/σ(I)> | 15.9 | 5.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 14.5 | 14.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 2.0 M MgCl2 and 100 mM Bicine (pH 9.0) |
