5XHA
Aspergillus kawachii beta-fructofuranosidase complexed with fructose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.357, 108.357, 152.852 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.010 - 2.100 |
| R-factor | 0.18025 |
| Rwork | 0.179 |
| R-free | 0.20979 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xh8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.307 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.076 | 0.469 |
| Number of reflections | 61268 | |
| <I/σ(I)> | 36.1 | 4.8 |
| Completeness [%] | 99.8 | |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 0.1 M sodium acetate, 2.6 M sodium formate |
