5XH9
Aspergillus kawachii beta-fructofuranosidase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-06-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 108.786, 108.786, 153.196 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.010 - 2.300 |
R-factor | 0.19024 |
Rwork | 0.189 |
R-free | 0.21877 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5xh8 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.396 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.010 | 2.340 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.093 | 0.365 |
Number of reflections | 47061 | |
<I/σ(I)> | 24.5 | 0.365 |
Completeness [%] | 99.9 | |
Redundancy | 9 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M sodium phosphate, 2.6 M sodium formate |