5XGZ
Metagenomic glucose-tolerant glycosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-11-10 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 202.427, 202.427, 202.427 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.750 |
| R-factor | 0.20875 |
| Rwork | 0.206 |
| R-free | 0.25477 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jie |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.840 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 4.750 | 1.750 |
| Rmerge | 0.091 | 0.042 | 0.401 |
| Rmeas | 0.095 | 0.043 | 0.473 |
| Rpim | 0.027 | 0.009 | 0.243 |
| Total number of observations | 1151056 | ||
| Number of reflections | 134784 | 7104 | 6360 |
| <I/σ(I)> | 9.5 | ||
| Completeness [%] | 98.0 | 99.8 | 93.3 |
| Redundancy | 8.5 | 20.4 | 3.2 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl buffer, 1.3 M lithium sulfate, 0.01 M nickel chloride |
