5XGW

Isoaspartyl dipeptidase from Colwellia psychrerythraea strain 34H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-10-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 4 21 2
Unit cell lengths	108.001, 108.001, 156.236
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 1.850
R-factor	0.16497
Rwork	0.163
R-free	0.20117
RMSD bond length	0.022
RMSD bond angle	2.195
Data scaling software	HKL-2000
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall
Low resolution limit [Å]	50.010
High resolution limit [Å]	1.850
<I/σ(I)>	46
Completeness [%]	99.9
Redundancy	25.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Bis-Tris propane pH 7.0, 1.4 M sodium malonate