5XGU
Escherichia coli. RNase R
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL15A1 |
| Synchrotron site | NSRRC |
| Beamline | BL15A1 |
| Temperature [K] | 277 |
| Detector technology | CCD |
| Collection date | 2011-10-24 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.691, 120.863, 83.626 |
| Unit cell angles | 90.00, 91.61, 90.00 |
Refinement procedure
| Resolution | 29.671 - 1.846 |
| R-factor | 0.188 |
| Rwork | 0.187 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vnu |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.672 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 124382 | 48331 |
| <I/σ(I)> | 11.8 | 3.7 |
| Completeness [%] | 99.1 | 99.14 |
| Redundancy | 4.5 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298.15 | 8% v/v Tacimate, pH 6.0, 10 % w/v Polyethylene glycol 3,350 |
