5XGT
Crystal structure of the N-terminal domain of Staphylococcus aureus single-stranded DNA-binding protein SsbA at 1.82 angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13C1 |
| Synchrotron site | NSRRC |
| Beamline | BL13C1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2015-10-17 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.975 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 88.792, 88.792, 57.686 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.820 |
| R-factor | 0.19525 |
| Rwork | 0.193 |
| R-free | 0.23311 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1x3e |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.860 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.900 |
| High resolution limit [Å] | 1.820 | 1.820 |
| Rmerge | 0.075 | |
| Number of reflections | 23485 | |
| <I/σ(I)> | 27.47 | 4.2 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.6 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 22% PEG 4000, 100mM HEPES sodium salt pH 7.5, 100mM sodium acetate |
