5XGS
Crystal structure of human WBSCR16
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-07-17 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 111.020, 54.550, 140.473 |
| Unit cell angles | 90.00, 95.82, 90.00 |
Refinement procedure
| Resolution | 34.937 - 2.000 |
| R-factor | 0.1815 |
| Rwork | 0.180 |
| R-free | 0.21820 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.531 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.3.11) |
| Phasing software | CRANK |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 34.940 | 34.940 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.173 | 0.062 | 0.840 |
| Rmeas | 0.201 | 0.073 | 0.980 |
| Rpim | 0.103 | 0.039 | 0.502 |
| Total number of observations | 214273 | ||
| Number of reflections | 56417 | ||
| <I/σ(I)> | 6.5 | ||
| Completeness [%] | 99.1 | 98 | 98.3 |
| Redundancy | 3.8 | 3.5 | 3.8 |
| CC(1/2) | 0.987 | 0.995 | 0.531 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | HEPES, PEG 20000 |
