5XGS

Crystal structure of human WBSCR16

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-17
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	I 1 2 1
Unit cell lengths	111.020, 54.550, 140.473
Unit cell angles	90.00, 95.82, 90.00

Refinement procedure

Resolution	34.937 - 2.000
R-factor	0.1815
Rwork	0.180
R-free	0.21820
Structure solution method	MAD
RMSD bond length	0.002
RMSD bond angle	0.531
Data reduction software	MOSFLM
Data scaling software	Aimless (0.3.11)
Phasing software	CRANK
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.940	34.940	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.173	0.062	0.840
Rmeas	0.201	0.073	0.980
Rpim	0.103	0.039	0.502
Total number of observations	214273
Number of reflections	56417
<I/σ(I)>	6.5
Completeness [%]	99.1	98	98.3
Redundancy	3.8	3.5	3.8
CC(1/2)	0.987	0.995	0.531

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	HEPES, PEG 20000