Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2014-09-22 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 76.000, 76.000, 144.410 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.474 - 2.350 |
| R-factor | 0.222 |
| Rwork | 0.218 |
| R-free | 0.25580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e2x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.663 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.474 | 2.400 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.071 | 0.410 |
| Number of reflections | 18014 | |
| <I/σ(I)> | 14 | 3.4 |
| Completeness [%] | 98.6 | 97 |
| Redundancy | 4.8 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 200mM Calcium Acetate, 100mM Sodium Cacodylate pH 6.5, 7% PEG 8000 |
