5XFP
Binary complex of PHF1 and a double stranded DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2016-03-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 109.601, 110.291, 118.853 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.765 - 2.300 |
| R-factor | 0.2106 |
| Rwork | 0.209 |
| R-free | 0.24370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5xfn |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.641 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.123 | 0.820 |
| Number of reflections | 65188 | |
| <I/σ(I)> | 22.4 | 2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 9.2 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.1M Tris-pH 8.5, 25% PEG 3350, 0.2M Li2SO4, 10mM MgCl2 |
