5XF7

Crystal structure of human protein disulfide isomerase-like protein of the testis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-11-11
Detector	APEX II CCD
Wavelength(s)	0.987
Spacegroup name	P 32
Unit cell lengths	97.361, 97.361, 57.588
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.106 - 2.381
R-factor	0.2003
Rwork	0.197
R-free	0.23520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ekz 4nwy
RMSD bond length	0.008
RMSD bond angle	0.957
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.420
High resolution limit [Å]	2.380	2.380
Number of reflections	24401
<I/σ(I)>	20.6	2
Completeness [%]	99.8	100
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	289	21% (w/v) PEG 3350 and 0.1 M succinic acid, pH 7.0