5XF2
Crystal structure of SeMet-HldC from Burkholderia pseudomallei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-10-21 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.9793 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.953, 73.953, 74.880 |
| Unit cell angles | 108.38, 108.38, 108.02 |
Refinement procedure
| Resolution | 31.268 - 2.800 |
| R-factor | 0.2438 |
| Rwork | 0.243 |
| R-free | 0.25190 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.348 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.940 | 2.950 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.073 | 0.120 |
| Number of reflections | 30332 | 4415 |
| <I/σ(I)> | 15.5 | 8.6 |
| Completeness [%] | 96.8 | 97 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 296 | 0.1M HEPES pH 7.0, 20% (w/v) polyethylene glycol 3350, 8.5mM n-octyl-beta-d-thiomaltoside |
