5XEO
Crystal structure of a hydrogen sulfide-producing enzyme (Fn1220) from Fusobacterium nucleatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2009-12-16 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 116.822, 116.822, 99.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 63.500 - 2.030 |
| R-factor | 0.17041 |
| Rwork | 0.168 |
| R-free | 0.21259 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.551 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.500 | 2.080 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.061 | 0.239 |
| Number of reflections | 44852 | 3255 |
| <I/σ(I)> | 42.9 | 17.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 26.3 | 25.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 41% (v/v) PEG 600, 0.1M Hepes pH 7.5, 0.17M Calcium acetate |
