5XEF

Crystal structure of flagellar chaperone from bacteria

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	80
Detector technology	AREA DETECTOR
Collection date	2016-02-24
Detector	ADSC QUANTUM 1
Wavelength(s)	0.97973
Spacegroup name	P 62
Unit cell lengths	83.207, 83.207, 47.771
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	72.060 - 2.000
R-factor	0.1955
Rwork	0.194
R-free	0.22740
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.283
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	REFMAC (5.8.0103)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	72.060	50.000	2.050
High resolution limit [Å]	2.000	4.830	2.000
Rmerge	0.093	0.068	0.186
Rmeas	0.095	0.071	0.192
Rpim	0.023	0.017	0.046
Number of reflections	18292
<I/σ(I)>	10.5
Completeness [%]	100.0	99.9	100
Redundancy	18.4	15.7	18
CC(1/2)		0.999	0.995

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	1.5 M ammonium sulfate 4 % isopropanol