5XED
Heterodimer constructed from M61A PA cyt c551-HT cyt c552 and HT cyt c552-PA cyt c551 chimeric proteins
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-13 |
Detector | DECTRIS EIGER X 4M |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 32.088, 65.912, 35.380 |
Unit cell angles | 90.00, 103.72, 90.00 |
Refinement procedure
Resolution | 34.370 - 1.550 |
R-factor | 0.21016 |
Rwork | 0.209 |
R-free | 0.23758 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 351c 1ynr |
RMSD bond length | 0.020 |
RMSD bond angle | 1.993 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.044 | 0.533 |
Number of reflections | 21840 | 1164 |
<I/σ(I)> | 16.6 | 2 |
Completeness [%] | 99.5 | 96.3 |
Redundancy | 3.3 | 3.1 |
CC(1/2) | 0.998 | 0.755 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 100 mM MES containing 25% w/v PEG 6000 |