5XEC

Heterodimer constructed from PA cyt c551-HT cyt c552 and HT cyt c552-PA cyt c551 chimeric proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-12
Detector	RAYONIX MX225HE
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	31.350, 72.540, 35.010
Unit cell angles	90.00, 106.07, 90.00

Refinement procedure

Resolution	20.000 - 1.100
R-factor	0.17578
Rwork	0.175
R-free	0.19731
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	351c 1ynr
RMSD bond length	0.010
RMSD bond angle	1.408
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0073)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.160
High resolution limit [Å]	1.100	1.100
Rmerge	0.078	0.389
Number of reflections	60431	8875
<I/σ(I)>	7.8	2.7
Completeness [%]	99.3	100
Redundancy	3.5	3.5
CC(1/2)	0.997	0.843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	277	100 mM Tris-HCl containing 200 mM sodium acetate, 30% w/v PEG 4000