5XDZ

Crystal structure of zebrafish SNX25 PX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-01-05
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.9785
Spacegroup name	P 21 21 2
Unit cell lengths	68.150, 82.830, 36.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.627 - 1.700
R-factor	0.1912
Rwork	0.189
R-free	0.23710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pqo
RMSD bond length	0.013
RMSD bond angle	1.346
Data scaling software	Aimless (0.5.17)
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	68.150	68.150	1.730
High resolution limit [Å]	1.700	9.000	1.700
Rmerge	0.053	0.076	0.276
Rmeas	0.057	0.082	0.298
Rpim	0.020	0.028	0.108
Total number of observations	155855	1207	8275
Number of reflections	23068
<I/σ(I)>	12.6	24.1	2.5
Completeness [%]	98.7	96.9	99.2
Redundancy	6.8	6.2	6.8
CC(1/2)	0.998	0.991	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	6.5	293	1.6M Sodium citrate tribasic dihydrate pH 6.5