5XDZ
Crystal structure of zebrafish SNX25 PX domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-01-05 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 68.150, 82.830, 36.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.627 - 1.700 |
| R-factor | 0.1912 |
| Rwork | 0.189 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4pqo |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.346 |
| Data scaling software | Aimless (0.5.17) |
| Phasing software | MOLREP |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 68.150 | 68.150 | 1.730 |
| High resolution limit [Å] | 1.700 | 9.000 | 1.700 |
| Rmerge | 0.053 | 0.076 | 0.276 |
| Rmeas | 0.057 | 0.082 | 0.298 |
| Rpim | 0.020 | 0.028 | 0.108 |
| Total number of observations | 155855 | 1207 | 8275 |
| Number of reflections | 23068 | ||
| <I/σ(I)> | 12.6 | 24.1 | 2.5 |
| Completeness [%] | 98.7 | 96.9 | 99.2 |
| Redundancy | 6.8 | 6.2 | 6.8 |
| CC(1/2) | 0.998 | 0.991 | 0.969 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6.5 | 293 | 1.6M Sodium citrate tribasic dihydrate pH 6.5 |
