5XDM

Structure of the C-terminal domain of E. coli MinC at 3.0 angstrom resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-27
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00
Spacegroup name	P 43 2 2
Unit cell lengths	65.061, 65.061, 115.926
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.589 - 3.004
R-factor	0.2331
Rwork	0.229
R-free	0.27520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hf2
RMSD bond length	0.004
RMSD bond angle	0.710
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX ((1.10.1_2155))
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.589	3.110
High resolution limit [Å]	3.004	3.000
Number of reflections	7967
<I/σ(I)>	8.6
Completeness [%]	99.5
Redundancy	12.3	8.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.2	293.15	0.1 M Tris-HCl, pH 7.2, 0.44 M sodium/potassium L-(+)-tartrate