5XDD
Crystal structure of tertiary complex of TdsC from Paenibacillus sp. A11-2 with FMN and Indole
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-22 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 100.790, 100.790, 424.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.473 - 1.900 |
| R-factor | 0.1802 |
| Rwork | 0.178 |
| R-free | 0.21060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.050 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.500 |
| High resolution limit [Å] | 1.810 |
| Number of reflections | 353039 |
| <I/σ(I)> | 8.32 |
| Completeness [%] | 92.5 |
| Redundancy | 2.79 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 1.4 M ammonium sulfate, 0.1 M Tris-HCl, 12% (v/v) Glycerol |
