5XDB

Crystal structure of FMN-bound TdsC from Paenibacillus sp. A11-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	CCD
Collection date	2013-10-10
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	100.820, 100.820, 424.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.500 - 1.811
R-factor	0.1909
Rwork	0.190
R-free	0.21170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xb8
RMSD bond length	0.006
RMSD bond angle	0.805
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.510
High resolution limit [Å]	1.811
Number of reflections	527346
<I/σ(I)>	8.7
Completeness [%]	100.0
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	1.4 M ammonium sulfate, 0.1 M Tris-HCl, 12% (v/v) Glycerol