5XDA

Structural basis for Ufm1 recognition by UfSP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-10
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	C 2 2 21
Unit cell lengths	267.447, 455.326, 195.558
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.165 - 3.285
R-factor	0.207
Rwork	0.207
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ejj
RMSD bond length	0.003
RMSD bond angle	0.666
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.420
High resolution limit [Å]	3.300	3.300
Rmerge	0.114
Number of reflections	179483
<I/σ(I)>	0.114
Completeness [%]	96.6
Redundancy	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1M Bis-Tris propane, 1M tri-ammonium citrate