5XCE
Crystal structure of Wild type Vps29 from Entamoeba histolytica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-22 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 35.564, 78.771, 67.974 |
| Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
| Resolution | 31.534 - 1.860 |
| R-factor | 0.2004 |
| Rwork | 0.198 |
| R-free | 0.24710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w24 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.047 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.860 |
| Number of reflections | 31246 |
| <I/σ(I)> | 39 |
| Completeness [%] | 99.9 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 289 | 200 mM Sodium Acetate, 100mM Sodium cacodylate pH 6.5, 30% PEG 8K |
