5XC9

Crystal structure of GH45 endoglucanase EG27II at pH8.0, in complex with cellobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	293
Detector technology	CCD
Collection date	2016-10-18
Detector	RAYONIX MX225HE
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	35.021, 47.278, 92.695
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.116 - 1.280
R-factor	0.1066
Rwork	0.105
R-free	0.13230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wc2
RMSD bond length	0.010
RMSD bond angle	1.253
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.300
High resolution limit [Å]	1.280	1.280
Rmerge	0.064	0.454
Number of reflections	40517	1979
<I/σ(I)>	44	4.7
Completeness [%]	100.0	99.7
Redundancy	9.4	8.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG4000, 200 mM KCl, 50 mM MES, 10 mM Cellobiose