5XC8

Crystal structure of GH45 endoglucanase EG27II at pH5.5, in complex with cellobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	293
Detector technology	CCD
Collection date	2016-10-18
Detector	RAYONIX MX225HE
Wavelength(s)	1.00000
Spacegroup name	P 21 21 21
Unit cell lengths	34.853, 47.309, 93.081
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.541 - 1.450
R-factor	0.1068
Rwork	0.105
R-free	0.13810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wc2
RMSD bond length	0.013
RMSD bond angle	1.360
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.480
High resolution limit [Å]	1.450	1.450
Rmerge	0.075	0.585
Number of reflections	28078	1354
<I/σ(I)>	30.5	3.9
Completeness [%]	99.9	99.9
Redundancy	7.9	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG4000, 200mM KCl, 50mM MES, 10mM Cellobiose