5XC5

Crystal structure of Acanthamoeba polyphaga mimivirus Rab GTPase in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	93
Detector technology	CCD
Collection date	2016-12-15
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	32.924, 53.724, 96.482
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.160 - 1.398
R-factor	0.1748
Rwork	0.174
R-free	0.19020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xc3
RMSD bond length	0.006
RMSD bond angle	0.976
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.398
Number of reflections	33149
<I/σ(I)>	14.5
Completeness [%]	98.4
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M lithium nitrate, 20%(w/v) polyethylene glycol 3350