5XC4
Crystal structure of GH45 endoglucanase EG27II at pH4.0, in complex with cellobiose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 293 |
Detector technology | CCD |
Collection date | 2016-10-18 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.00000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 34.770, 47.318, 93.324 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.203 - 1.420 |
R-factor | 0.1142 |
Rwork | 0.113 |
R-free | 0.14810 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wc2 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.290 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.440 |
High resolution limit [Å] | 1.420 | 1.420 |
Rmerge | 0.071 | 0.598 |
Number of reflections | 29924 | 1463 |
<I/σ(I)> | 29 | 2.9 |
Completeness [%] | 99.9 | 98.3 |
Redundancy | 7.2 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 25% PEG4000, 200mM KCl, 50mM MES, 10mM Cellobiose |