5XBZ
Crystal structure of GH family 81 beta-1,3-glucanase from Rhizomucr miehei complexed with laminaripentaose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BSRF BEAMLINE 3W1A |
| Synchrotron site | BSRF |
| Beamline | 3W1A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-20 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.916, 96.958, 127.642 |
| Unit cell angles | 90.00, 93.91, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.700 |
| R-factor | 0.17777 |
| Rwork | 0.174 |
| R-free | 0.19047 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4k3a |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.669 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.700 |
| Number of reflections | 35201 |
| <I/σ(I)> | 8.66 |
| Completeness [%] | 99.1 |
| Redundancy | 15 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1%(w/v) laminaripentaose, 24%(w/v) PEG4000 , 80mM Tris-HCl (pH 8.5), 6%(v/v) MPD |
