5XBX

Crystal structure of GH45 endoglucanase EG27II in complex with cellobiose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2016-05-27
Detector	RIGAKU RAXIS V
Wavelength(s)	0.8000
Spacegroup name	P 21 21 21
Unit cell lengths	34.472, 46.499, 92.014
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.501 - 1.040
R-factor	0.1239
Rwork	0.123
R-free	0.13870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wc2
RMSD bond length	0.010
RMSD bond angle	1.240
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.060
High resolution limit [Å]	1.040	1.040
Rmerge	0.051	0.390
Number of reflections	71762	3335
<I/σ(I)>	49.3	4.9
Completeness [%]	99.6	93.8
Redundancy	6.3	5.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	25% PEG4000, 200 mM KCl, 50 mM MES, 10 mM Cellobiose, Soaking in 255 mM Cellobiose