5XBV
Crystal structure of Wss1 mutant from saccharomyces cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-10 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 61 |
| Unit cell lengths | 86.202, 86.202, 36.287 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 24.884 - 1.804 |
| R-factor | 0.1744 |
| Rwork | 0.173 |
| R-free | 0.20030 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.833 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.063 | 0.506 |
| Rpim | 0.021 | 0.171 |
| Number of reflections | 14399 | 702 |
| <I/σ(I)> | 35.98 | 3.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.9 | 9.7 |
| CC(1/2) | 0.922 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10%(w/v) PEG-8000, 20mM Tris-HCl, pH 7.0, and 0.2M MgCl2 |
