5XBS
Peroxiredoxin from Aeropyrum pernix (6m mutant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-03 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.656, 170.788, 61.972 |
| Unit cell angles | 90.00, 114.00, 90.00 |
Refinement procedure
| Resolution | 36.330 - 2.510 |
| R-factor | 0.21476 |
| Rwork | 0.210 |
| R-free | 0.30048 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.553 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 28181 |
| <I/σ(I)> | 14.2 |
| Completeness [%] | 84.8 |
| Redundancy | 4.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.1 M sodium cacodylate (pH 6.5), 0.2 M magnesium acetate, and 20% PEG8000 |
