5XBR
Peroxiredoxin from Pyrococcus horikoshii (sulfonic acid form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-09-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 6 |
| Unit cell lengths | 140.466, 140.466, 48.023 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.640 - 2.100 |
| R-factor | 0.19214 |
| Rwork | 0.189 |
| R-free | 0.24379 |
| RMSD bond length | 0.025 |
| RMSD bond angle | 1.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 40.000 |
| High resolution limit [Å] | 2.100 |
| Number of reflections | 31183 |
| <I/σ(I)> | 11.4 |
| Completeness [%] | 98.3 |
| Redundancy | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 293 | 0.1 M sodium citrate (pH 5.4), 0.2 M potassium sodium tartrate, and 1.5 M ammonium sulfate |
