5XBK

Crystal structure of human Importin4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	110
Detector technology	CCD
Collection date	2016-09-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97950
Spacegroup name	P 1 21 1
Unit cell lengths	65.021, 108.536, 124.860
Unit cell angles	90.00, 103.41, 90.00

Refinement procedure

Resolution	49.977 - 3.223
R-factor	0.2246
Rwork	0.218
R-free	0.28090
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xah
RMSD bond length	0.004
RMSD bond angle	0.672
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.360
High resolution limit [Å]	3.200	3.200
Rmerge	0.102
Rmeas	0.110
Rpim	0.041
Total number of observations	247205
Number of reflections	26893
<I/σ(I)>	12.9	2.34
Completeness [%]	99.2	100
Redundancy	7.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Sodium tartrate, PEG3350