5XB0

1.6 A crystal structure of peptidyl-prolyl cis-trans isomerase PPIase from Pseudomonas syringae pv. tomato str. DC3000 (PSPTO DC3000)

Replaces:  3OE2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E DW
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2009-08-20
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	33.222, 44.099, 82.312
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.535 - 1.600
R-factor	0.1711
Rwork	0.168
R-free	0.19750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1fd9
RMSD bond length	0.006
RMSD bond angle	1.081
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.170	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.052	0.269
Number of reflections	16027	1182
<I/σ(I)>	49.24	5.8
Completeness [%]	96.5	96.6
Redundancy	11.8	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	7.3	291	0.4M pPotassium sodium tartrate