5XAZ
Crystal structure of full length native tylp, a tetr regulator from streptomyces fradiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-20 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9686 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.479, 71.566, 158.584 |
| Unit cell angles | 90.00, 102.92, 90.00 |
Refinement procedure
| Resolution | 19.810 - 2.300 |
| R-factor | 0.20451 |
| Rwork | 0.204 |
| R-free | 0.25258 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.074 |
| Data scaling software | Aimless |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.810 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.407 | |
| Number of reflections | 98807 | |
| <I/σ(I)> | 2.8 | 2.8 |
| Completeness [%] | 99.7 | |
| Redundancy | 3.5 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.2M Ammonium citrate tribasic (pH 7.0), 20% w/v PEG 3350 |
