5XAX

Parallel homodimer structures of the extracellular domains of the voltage-gated sodium channel beta4 subunit explain its role in cell-cell adhesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44B2
Synchrotron site	SPring-8
Beamline	BL44B2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-12-22
Detector	MAR CCD 165 mm
Wavelength(s)	0.8
Spacegroup name	I 2 3
Unit cell lengths	131.545, 131.545, 131.545
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.157 - 2.903
R-factor	0.252
Rwork	0.247
R-free	0.29940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ayq
RMSD bond length	0.005
RMSD bond angle	0.978
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	35.200
High resolution limit [Å]	2.900
Number of reflections	8526
<I/σ(I)>	31.4
Completeness [%]	99.6
Redundancy	7.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	2.7M Na-malonate, 0.1M Tris-HCl (pH 7.0), 0.5 % (v/v) Tween 20