5XAW

Parallel homodimer structures of voltage-gated sodium channel beta4 for cell-cell adhesion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2009-10-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1
Spacegroup name	P 32
Unit cell lengths	57.079, 57.079, 72.024
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.107 - 2.101
R-factor	0.2102
Rwork	0.204
R-free	0.26390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5ayq
RMSD bond length	0.007
RMSD bond angle	0.964
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	29.107
High resolution limit [Å]	2.100
Number of reflections	14834
<I/σ(I)>	32.5
Completeness [%]	97.4
Redundancy	6.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		293	10% (w/v) PEG1000, 10% (w/v) PEG 8000