5XAV
Structure of PhaC from Chromobacterium sp. USM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-19 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 61 |
| Unit cell lengths | 117.332, 117.332, 105.911 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 36.105 - 1.479 |
| R-factor | 0.123 |
| Rwork | 0.121 |
| R-free | 0.15740 |
| Structure solution method | SAD |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.603 |
| Phasing software | autoSHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.510 |
| High resolution limit [Å] | 1.479 | 1.480 |
| Rmerge | 0.644 | |
| Number of reflections | 137720 | |
| <I/σ(I)> | 58.6 | 6.2 |
| Completeness [%] | 99.8 | |
| Redundancy | 11.2 | 11.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 277.15 | 50 mM Bis-Tris pH5.5, 60 mM Ammonium sulfate 5% PEG4000 |
