5XAJ
Structural mimicry of the dengue virus envelope glycoprotein revealed by the crystallographic study of an idiotype-anti-idiotype Fab complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2016-07-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0428 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 116.300, 47.590, 220.759 |
| Unit cell angles | 90.00, 93.43, 90.00 |
Refinement procedure
| Resolution | 46.930 - 2.500 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.23900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Phasing software | MOLREP |
| Refinement software | BUSTER (2.10.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.670 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 84640 | 12224 |
| <I/σ(I)> | 6.5 | 1.6 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | Ammonium acetate, Bis Tris Propane pH 5.5, PEG 10,000 |
