5XAH

Crystal structure of human Importin4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	110
Detector technology	CCD
Collection date	2014-11-14
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.980001
Spacegroup name	P 1 21 1
Unit cell lengths	64.920, 110.769, 126.820
Unit cell angles	90.00, 106.50, 90.00

Refinement procedure

Resolution	46.623 - 3.004
R-factor	0.2311
Rwork	0.226
R-free	0.28030
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.739
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.050
High resolution limit [Å]	3.000	8.130	3.000
Rmerge	0.102	0.070	0.599
Rmeas	0.110	0.076	0.652
Rpim	0.041	0.028	0.253
Total number of observations	247205
Number of reflections	33901
<I/σ(I)>	12.9
Completeness [%]	99.2	99.7	97.5
Redundancy	7.3	7.2	6.6
CC(1/2)		0.995	0.798

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		291	Sodium Tartrate, PEG3350