5XA9
Complete structure factors and an atomic model of the calcium pump (SERCA1A) and associated phospholipids in the E2-ALF-(TG) crystals of C2 symmetry
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-07-13 |
| Detector | RIGAKU RAXIS V |
| Wavelength(s) | 0.8 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 177.400, 70.100, 141.900 |
| Unit cell angles | 90.00, 107.00, 90.00 |
Refinement procedure
| Resolution | 135.700 - 3.200 |
| R-factor | 0.216 |
| Rwork | 0.215 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WPE |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.865 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 135.700 |
| High resolution limit [Å] | 3.200 |
| Number of reflections | 27887 |
| <I/σ(I)> | 49.78 |
| Completeness [%] | 100.0 |
| Redundancy | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6.1 | 283 | PEG 400 |
