5XA2
Crystal Structure of O-acetylserine sulfhydrylase from Planctomyces Limnophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-05-17 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 54.996, 90.041, 128.360 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.620 - 2.034 |
| R-factor | 0.1626 |
| Rwork | 0.160 |
| R-free | 0.20470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ho1 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.902 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.620 | 2.070 |
| High resolution limit [Å] | 2.034 | 2.030 |
| Rmerge | 0.050 | 0.273 |
| Number of reflections | 37925 | |
| <I/σ(I)> | 30.1 | 3.5 |
| Completeness [%] | 90.5 | |
| Redundancy | 6.4 | 3.5 |
| CC(1/2) | 1.000 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 20% PEG 8000 100mM Tris pH8.5 200mM MgCl2 |
